PRIYAWAN RACHMADI, MUHAMMAD LUTHFI,*, AQSA SJUHADA OKI, MIEKE SYLVIAMAR AND MUHAIMIN RIFAI
Department of Dental Material, Faculty of Dentistry, Universitas Airlangga, Surabaya 60285, Indonesia
*(email : m.luthfi@fkg.unair.ac.id, Phone : +6281357898957)
ABSTRACT
Dental caries is a chronic, multifactorial disease that begins with a microbiological shift in complex biofilms (dental plaque) that is influenced by consumption of sugar, salivary flow, and behaviour. The present investigation was undertaken to analyze the expression of pro-inflammatory cytokines TNF-α on salivary neutrophil surfaces using flow cytometer. Extra Centella asiatica reduced pro-inflammatory cytokines
TNF-α on the neutrophil surface of salivary free caries at 0 µg/ml (24.04±0.70), 12.5 µg/ml (21.88±1, 48), 25 µg/ml (20.56±0.95), 50 µg/ml (19.28±1.32), 100 µg/ml (18.07±1.17), 200 µg/ml (17.28±0.84), while in
S-ECC at 0 (35.95±3.50), 12.5 (28.88±1.32), 25 (27.82±1.28), 50 (26.46±2.95) and 100 (23.46±1.42). C. asiatica decreased pro-inflammatory cytokines TNF-α produced by salivary neutrophils.
Key words : Severe early childhood caries, neutrophil cells, TNF-α pro-inflammatory cytokines