ARVIND MALIK*, RACHNA GULATI, KOMAL DUHAN AND SURJEET SINGH
Department of Zoology, CCS Haryana Agricultural University, Hisar-125 004 (Haryana), India
*(e-mail : email@example.com)
(Received : December 23, 2017; Accepted : February 19, 2018)
The present research evaluates the efficacy of methanolic leaf extract of Pongamia pinnata by two bioassays : direct spray and treated bioassay under laboratory conditions at eight concentrations (0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0%) against Tyrophagus putrescentiae. Efficacy against mites increased significantly with increasing concentrations in both the bioassays. Each bioassay of plant extract revealed a significant and positive role in mite population inhibition. Direct spray bioassay caused high reduction of population (41.33 to 76.00%) as compared to treated bioassay (28.00 to 63.33%). Direct spray bioassay showed lower LC50 value (0.629 ml/100 ml) as compared to treated bioassay (0.638 ml/100 ml). This study highlights an opportunity to develop natural molecules from P. pinnata leaf extracts as acaricides.
Key words : Bioassay, LC50, mites, Pongamia pinnata, Tyrophagus putrescentiae