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Field Evaluation and Microsatellite Marker Analysis of IB370 × MAS25/ IB370 and IB370 × MASARB-25/ IB370 BC1F1 Plants under Direct-seeded Aerobic Field Conditions

RAJESH YOGI, NAVEEN KUMAR*, RAHUL KUMAR MEENA AND RAJINDER KUMAR JAIN
Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University,
Hisar-125 004 (Haryana), India
*(e-mail : nimbhal@gmail.com; Mobile : 9996349056)
(Received : February 19, 2019; Accepted : April 10, 2019)

ABSTRACT

Increasing water scarcity has threatened the cultivation of lowland Basmati varieties in northern states of
India including Haryana. Marker assisted selection can greatly improve the efficacy of Basmati rice breeding
programmes. Molecular marker technology can help us in identification of suitable parental lines, diversity
and pedigree analyses and to introgress desirable traits without altering the genetic background. In the
present investigation, experiments were conducted to evaluate BC1F1 plants derived from crosses between Basmati (Improved Basmati 370, IB370) and aerobic rice varieties (MAS25 and MAS-ARB25), for various
physio-morphological traits under aerobic conditions in field. IB370 × MAS25/IB370 and IB370 × MASARB25/IB370 derived BC1F1 plants showed variation for plant height (99.0-130.0 and 100-120 cm), panicle
length (23.3-26.4 and 22.6-27.2 cm), effective number of tillers per plant (5-18 and 7-21), length/breadth
ratio of dehusked grain (3.4-4.5 and 3.4-4.4), grain yield per plant (11.7-30.3 and 15.2-34.5 g) and 1000-
grain weight per plant (20.8-22.9 and 18.3- 23.4 g), respectively.

Key words : Field evaluation, microsatellite marker analysis, BC1F1 plants, direct-seeded, aerobic