R. BLIEVA*, ZH. SULEIMENOVA, G. ULTANBEKOVA, N. AKMUKHANOVA, N. ALTYBAEVA AND A. NURMAHANOVA
Research and Production Enterprise Antigen Co. Ltd., Almaty, Kazakhstan
*(e-mail : email@example.com; Mobile : +77077259059)
(Received : May 22, 2019; Accepted : July 18, 2019)
Collagenases are among the most important hydrolytic enzymes which have great potential in various industrial processes such as leather, detergent, textile, etc. Although many microorganisms produce this enzyme, in the recent period the most commonly used for their industrial application are Aspergillus fungi. Among media components, the carbon and nitrogen sources are the valuable nutritional factors for costefficient enzyme production. The aim of the present study was the selection of suitable carbon and nitrogen sources of Aspergillus awamori 16 and Aspergillus awamori 22 mixed cultures for maximal production of extracellular protease. Sucrose (4.2 U/ml) and peptone (4.8 U/ml) were found as the best carbon and nitrogen sources, respectively.
Key words :Collagenase, Aspergillus fungi, carbon sources, nitrogen sources