SALAMUN*, NI’MATUZAHROH, FATIMAH, VICKY FINDAWATI, RIZKY DANANG
SUSETYO, NADIAH AL-BATATI, TRI NURHARIYATI AND AGUS SUPRIYANTO
Laboratory of Microbiology, Department of Biology, Faculty of Science and Technology, University of Airlangga,
Jl. Dr. Ir. Soekarno, Surabaya, Indonesia 60115
*(e-mail : salamun@fst.unair.ac.id; Mobile : +6281332198122)
ABSTRACT
The research was aimed at the exploration of native entomopathogenic Bacilli from natural breeding sites of Dengue Fever Vector in Baluran National Park, East Java, Indonesia. Soil samples were collected from
30 sites at three different locations. Isolation of bacteria used selected growth media for Bacillus sp. and characterized based on spore staining method. The isolates tested the mortality rates against Aedes aegypti larvae and observed 24 and 48 h after exposure. The potential isolates status was based on the mortality rates (%) of Ae. aegypti larvae. Furthermore, the characterization of macroscopic and microscopic and physiologic was identified as species name of Bacillus sp. by similarity index. The results of the initial potential test showed that 68 of 107 isolates of Bacillus sp. were obtained as native entomopathogenic Bacillus sp. with variations of the potential status. In the advanced potential test, there were three isolates with highest potential status, coded as BK7.1, BK7.2 and BK5.2, with the mortality rates 93, 87 and 70%, respectively. Based on macroscopic, microscopic and physiological characterizations Bacillus sp. BK7.1 had similarity with Bacillus sphaericus and Bacillus sp. BK7.2 had similarity with Bacillus subtilis and Bacillus sp. BK5.2 isolate had similarity with Bacillus thuringiensis.
Key words : Entomophatogenic, Bacillus sp., Aedes aegypti, breeding sites, Baluran National Park