A. D. PUTRI AMBARSARI, HADIWIYONO, SALIM WIDONO AND AHMAD YUNUS
* Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Ir. Sutami Street 36 A, Surakarta (Solo) 57126, Indonesia
*(e-mail : email@example.com; Mobile : 6281548673060)
(Received : May 12, 2019; Accepted : August 14, 2019)
Plant disease appearance can cause an important problem in rice cultivation. One of the rice diseases is a rice sheath blight caused by Rhizoctonia solani AG1-IA. Endophyitic bacteria from medicinal plant artemisia as a biocontrol agent need to be considered. This study aimed at studying the capability of endophytic bacteria from medicinal plant Artemisia to inhibit the growth of R. solani through in vitro and in vivo test. The method of this study was isolation and morphology characterization of endophytic bacteria colony, in vitro antagonism test, and in vivo antagonism test that was conducted using a complete randomized design (CDR) with six treatments and each treatment was replicated four times. The results showed that there were 12 endophytic bacterial isolates that were successfully isolated. Only five endophytic bacteria isolates were used for subsequent test. BEA 12 isolate showed the highest in vitro antagonistic activity (49.17%) against R. solani. The inhibition of endophytic bacterial isolates against R. solani could be caused due to antifungal activity in the form of enzymes or antibiotics produced by endophytic bacteria. Meanwhile, the result of in vivo test showed that BEA 12 was more effective to control the disease.
Key words :Biological control, antagonism, Rhizoctonia solani