Andriyana Setyawati *, Edi Purwanto, Retno Bandriyati Arni Putri, Lintang Astri Widowati and Tilmiidzah Salma Fathin
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret (UNS), Jl. Ir. Sutami 36A, Kentingan, Surakarta 57126, Indonesia
*(e-mail: andriyanasetyawati@staff.uns.ac.id; Mobile: +6285293942727)
(Received: 8 February 2025; Accepted: 27 May 2025)
ABSTRACT
Cultivation of the raja bulu banana (Musa paradisiaca var. raja bulu) is carried out on a large scale through tissue culturing. However, the slow growth of raja bulu banana seeds necessitates the use of PGRs to improve the growth of the cultured explants. This optimization was studied at the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, UNS Surakarta, April–August 2024. A completely randomized study design was used with two factors: the concentrations of jasmonic acid (JA) and benzylaminopurine (BAP). The same concentrations were used (0, 0.5, 1, and 1.5 ppm) with three repetitions. Observational data were analyzed using analysis of variance, and if the treatments were significantly different, then processed by DMRT at a 5% significance level. The results showed that a combination of JA and BAP in MS media could increase the root lengths of Raja Bulu banana subcultures. The number of leaves could be increased in the absence of additional JA. JA at 0.5 ppm shortened the time of root emergence by 15.9%, and at 1 ppm shortened the time of shoot emergence by 8.92%. BAP at 1.5 ppm could increase the number of shoots of Raja Bulu banana subcultures by 64.72%. JA at 1 ppm and BAP at 0 ppm was the best combination for increasing the length of the roots of Raja Bulu banana subcultures, by up to 12.12%.
Key words : cytokinin, horticultural plants, plant hormone, tissue culture