POOJA BANGAR*, ASHOK CHAUDHURY1, BHAVANA TIWARI, SANJAY KUMAR, RATNA KUMARI AND K.V. BHAT
Genomic Resources Division, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-
110 012, India
*(e-mail : tulip.bangar@gmail.com; Mobile : 9582341829)
(Received : December 17, 2018; Accepted : February 18, 2019)
ABSTRACT
The study was conducted to identify SNPs suitable for analysis of genetic diversity and establish robust
SNP marker profile for identification of mungbean varieties. The extent of polymorphism detected with the
presently available co-dominant markers such as simple sequence repeats was much limited. Hence,
single nucleotide polymorphism (SNP) markers that provide a promising means for developing rapid,
automated and high throughput molecular profiles were used. Here, a novel set of five SNP regions were
identified by analysing a large number of SNP markers developed using whole genome re-sequencing
approach for discriminating a set of 34 selected mungbean varieties. Polymorphism information content
(PIC) and major allele frequency (MAF) ranged from 0.08 to 0.21 (an average of 0.16) and 0.86 to 0.95 (an
average of 0.90), respectively. The results indicated the presence of low genetic distance between the
mungbean varieties. These novel SNP markers will allow rapid and accurate identification of mungbean
varieties and will find application in marker assisted mungbean breeding programmes.
Key words :Mungbean, single nucleotide polymorphism, simple sequence repeats, DNA fingerprinting,genetic diversity