BASHAIK RAHANA PARVEEN, SHEETU WADHWA*, SACHIN KUMAR SINGH**, VANCHA
HARISH LEANDER CORRIE AND ANKIT AWASTHI
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara-144 411 (Punjab), India
*(e-mail :sheetupharma@gmail.com; Mobile : 70877 32988 and **singhsachin23@gmil.com; Mobile : 98887 20835)
(Received: October 29, 2022; Accepted: December 3, 2022)
ABSTRACT
A simple analytical UV-visible spectrophotometric method was developed and validated. For that central
composite design (CCD) was used to optimize a UV-visible spectrophotometer method for analysis of
chrysin (CS). The sample interval and scanning speed were selected as the critical method variables
(CMV). The CCD produced the 13 runs for optimization of CMV. Based upon the obtained response
results the optimum condition of sample speed and sample interval selected was -0.60 nm/sec and
-0.51 nm, respectively. The calibration curve of CS was plotted for concentration range of 2-10 µg/ml.
The method was validated for its linearity, precision, accuracy, limit of detection (LOD) and limit of
quantification (LOQ) as per ICH Q2 (R1) guidelines. The developed method for CS was found to be linear
in the concentration range of 2-10 µg/ml with high correlation coefficient of 0.999. The mean percentage
recovery was in the range of 97-98% which confirmed the accuracy of the method. The relative standard
deviation of CS for precision was less than 2% which indicated that method was precise. The LOD and
LOQ of CS were found to be 0.277 and 0.84 µg/ml, respectively. The percentage entrapment efficiency
(%EE) and (%DL) of CS in formulation (F5) was found to be 90±0.16 and 7.5±0.15%, respectively. Therefore,
the method was effectively used for the estimation of CS in the bulk and also in nanostructured lipid
carriers (NLCs).
Key words : CS, accuracy, central composite design, sample interval, nanostructured lipid carriers