Mousa Atallah Altarawneh, Haitham Qaralleh, Ahmad M. Al-Jaafreh, Hammad Al Dal’in, Muhamad O. Al-limoun, Jawad A. Al-dalaeen, Qutaibah Al_btoush and Samar Janel Salahat
Department of Medical Support, Al-Karak University College, Al-Balqa Applied University, Al Karak 61710, Jordan
1Department of Medical Laboratory Sciences, Mutah University, Mutah, Al-Karak 61710, Jordan.
2Department of Biological Sciences, Faculty of Science, Mutah University, P.O. Box 7, Mutah 61710, Jordan.
3Deparment of Financial and Administrative Sciences, Al-Karak University College, Al-Balqa Applied University, Al-Karak 61710, Jordan
*(e-mail: m.altarawneh@bau.edu.jo; Mobile: 962 7 9880 6816)
(Received: February 2, 2024; Accepted: April 10, 2024)
For AOABR
ABSTRACT
The present study reported the phytochemical profile, LCMS analysis, and antibacterial and antioxidant activities of
Carpobrotus edulis. The phytochemical analysis confirmed that the highest total phenolic components (TPC) and total flavonoid components (TFC) were extracted by ethanol (208.73 mg GAE/g extract and 73.33 mg TE/g extract, respectively), while the highest total tannic components (TNC; 62.71 mg GAE/g extract) was extracted by ethyl acetate. The LCMS analysis revealed that catechin was the most prevalent compound in the hexane extract, accounting for 18.0%. In contrast, quercetin, procyanidin, and quercetin were the prominent components in the ethyl acetate, ethanol, and water extracts, respectively, with 8.8, 15.2, and 11.8%. The antioxidant potential of C. edulis using DPPH and ABTS assays showed that the maximum scavenging power was observed for water extract, followed by ethanol, ethyl acetate, and then hexane. The antibacterial evaluation presented C. edulis extracts as a broad-spectrum antibacterial agent. Water, ethanol, ethyl acetate, and hexane were the most effective extracts against the tested bacteria. However, taphylococcus aureus was the most susceptible bacteria since it exhibited the highest inhibition zone (19.0 mm) and the lowest MIC values (0.3 mg/mL). The antioxidant and antibacterial properties of C. edulis extracts were influenced by their polarity. The water extract exhibited the highest levels of antibacterial and antioxidant activity, which supported the rationale use of this plant in traditional medicine.
Key words : Carpobrotus edulis, antibacterial, antioxidant, chemical composition, LCMS