SHALIMA SIHAG, SUBHASH KAJLA*, ANIL K. POONIA AND JOGINDER SINGH DUHAN
Centre for Plant Biotechnology, CCS Haryana Agricultural University, Hisar-125 004 (Haryana), India
*(e-mail : ksubhash73@hotmail.com)
(Received : October 28, 2017; Accepted : December 12, 2017)
ABSTRACT
The present investigation was carried to study the effect of different carbon sources and gelling agents for improvement in potential micropropagation of Aloe vera. Shoot cultures were initiated on MS medium containing 2.0 mg/l BAP. The regenerated shoots were transferred on MS medium+2.0 mg/l BAP+0.5 mg/l NAA supplemented with various carbon sources and gelling agents. The maximum (24.9) shoots were observed on MS medium+2.0 mg/l BAP+0.5 mg/l NAA supplemented with 30 g/l sucrose. Table sugar was also at par with sucrose with 24.6 shoots on 30th day of sub-culture. The agar-agar @ 8.0 g/l was found to be most effective amongst the different gelling agents used with maximum (24.8) shoots on 30th day of sub-culture. The sucrose was found most effective carbon source for in vitro multiplication of Aloe vera with 24.9 shoots, while table sugar was also at par with sucrose. Therefore, for commercial purpose, table sugar is recommended instead of sucrose in the medium for cost effective in vitro propagation of Aloe vera. The elongated shoots responded maximum (100%) on MS medium (half strength) supplemented with 2.0 mg/l IBA on 10th day of inoculation. Rooted plants were hardened using different types of potting mixture of sand, soil and vermicompost for 30-40 days and sand+soil+vermicompost (1 : 1 : 1) was found best for survival of in vitro raised plants. Hardened plants were successfully transferred to field with 100% survival.
Key words : Micropropagation, Aloe vera, multiplication hardening