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Histological Effects of Titanium Dioxide Nanoparticles on Some Reproductive Organs in Male Mice (Mus musculus)

College of Science for Women, University of Babylon, Iraq
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(Received: May 15, 2022; Accepted: July 18, 2022)


The aim of our article was to investigate nanoparticles of TiO2 on the histology of some reproductive organs and some reproductive hormones levels in male albino mice orally administered with those nanoparticles. Thirty adult healthy male albino mice weighting 25-30 g and aged 12-15 weeks were
divided into three groups. First group of mice was given normal saline orally for 90 days. Second and
third groups were given 100 and 150 mg/kg body weight of TiO2 orally, respectively, for 90 days. The administration of nanoparticles of TiO2 decreased body weight and testosterone level of all treated mice and LH, FSH level increased the oxidative stress comparing with mice of control group. Histologically, it was found that there was marked alteration in organs: testis epididymis and seminal vesicles histologic architecture such as necrosis, hyperplasia, atrophy, arrested spermatogenesis. In conclusion, nanoparticles of TiO2 had reproductive toxicity on the male reproductive system of mice.
Key words : Nanoparticles of TiO2, reproductive system, histology, male mice