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Isolation and Identification of Alkaloid Compounds from Capparis spinosa and to Study its Biological Activity in Misan, Iraq

Department of Biology, College of Science, University of Misan, Iraq
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(Received: May 17, 2022; Accepted: June 19, 2022)


The current study included the preparation of alcoholic (methanol and ethanol) and aqueous extracts of
indigenous medicinal plant in Iraq. The leaves, stems and roots of the Capparis spinosa were used for
studying the chemical components of these extracts by using appropriate reagents. This study also
included the separation, purification and identification of the alkaloid compounds separated from the
roots of the C. spinosa plant. The separated alkaloids were diagnosed through the use of some physical
and chemical tests, which represented the degree of melting point and solubility, as well as gas
chromatography-mass spectroscpy, thin layer chromatography, ultraviolet spectrometry (UV) and fourier
transform infrared spectrometry (FT-IR). Antibacterial activity of the isolated alkaloid compounds against
harmful bacteria was demonstrated (Staphylococcus aureus and Escherichia coli ). Bacterial strains were
isolated from inflammation of the urinary tract (UTI) samples, and normal bacteriological procedures
were used to evaluate each bacterial isolate’s biochemical values. Using the Vitek-2 method and molecular
anlysis, the isolates were further identified.
Key words : Capparis spinosa, Staphylococcus aureus, Escherichia coli, Vitek-2 method