HIBA A. NASEAR*, SAMAR S. GHAZI, JALAL Y. MUSTAFA, ALAA Q. SADIQ AND MUSTAFA E. SHABAN
Department of Veterinary Public Health, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
*(e-mail: heba.naser@uobasrah.edu.iq; Mobile : +964 77255 69661)
(Received: December 15, 2022; Accepted: January 21, 2023)
ABSTRACT
The current study was aimed at isolating and molecular characterization of Hafnia alvei implicated in pathogenic list of mastitis in cows and buffaloes milk. Six (20%) and four (13.33%) isolates of Hafnia alvei
were obtained from raw milk of cows and buffaloes, respectively. The differences between cows and buffalo milk crude were not statistically significant. In the molecular diagnosis, the positive results for cows and buffaloes, respectively, were 5 (16.66%) in cows and 3 (10%) samples in buffaloes when diagnosed with the RB89-F/RB90-R gene. It was clearly shown with the 450 base pair gene.
Key words : Isolate, Hafnia alvei, cows, buffaloes