Effect of rice bran husk substrate on Production of Asparginase by Streptomyces sp adopted from termite mound

Abstract

An important criterion for the potency assessment is the source of cheap substrate on L-asparaginase production is much needed. Isolation of novel actinobacteria from termite mound was performed by conventional pre treatment isolation and enzyme screening by plate assay method. Effect of carbon on   L- asparaginase activity was done by submerged state by cultivating in modified minimal medium. Effected of rice bran husk used as alternate for carbon and enzyme production was studied by nesslerization method. Heat treated samples resulting reduced bacterial colonies but not inhibited. Out ten pre heat treated samples one soil showed existence of actinobacteria and nine were negative. The isolate found to be asparaginase positive designated as TS5 morphologically showed aerial and substrate mycelia formation and identified as Streptomyces sp. Production of L-asparaginase among four different carbon reveals that lactose found to be inducer. Further the cheap substrate such as rice bran is evaluated under substrate state fermentation and a good result on enzyme production is noted on both defatted and raw rice bran. The highest peak activity of the enzyme production (183±9.11 U/ml) was attained at 10 % raw rice bran with 66.3±0.26 mg/mL protein. These results suggest that increasing the levels of substrate and glucose within the studied ranges led to a significant increase in LA production. The results of these findings have promote attraction and increas the scope of finding industrially important actinomycetes from termite mound