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MDA and GSH Levels in the Blood Plasma of STZ-induced Diabetic Rats after Snakehead Fish (Channa striata) Extract Treatment

Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, 60111
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Snakehead fish extract (SHE) has high amino acid content which is precursor for glutathione (GSH). Glutathione is an endogenous antioxidant reduces oxidative stress. The present study was to determine the effect of SHE on MDA and GSH levels plasma of diabetic rats model induced by streptozotocin (STZ). There were five groups of treatments : Non-diabetic rats/negative control (C-), diabetic rats/positive control (C+), and 3 SHE levels of administration : 2 ml/day (low dose : LD), 3 ml/day (median dose : MD) and 4 ml/day (high dose : HD). The blood glucose level of normal rats (C-) was significantly lowered (P<0.05) than C+. However, only HD treatment showed significantly lowered blood glucose level (P<0.05) compared to C+. This result indicated that SHE treatment could not swiftly lower blood glucose levels down to the normal level. Furthermore, the glucose level after SHE treatment and MDA levels in plasma of diabetic rats without SHE treatment (C +) were significantly higher (P<0.05) than non-diabetic rats (C-)/normal rats and diabetic
rats treated with SHE (LD, MD and HD). Higher MDA levels in diabetic rats indicated oxidative stress in diabetic rats. Lower MDA levels (P<0.05) after SHE treatment showed a decrease in oxidative stress in diabetic rats and were statistically the same as MDA in normal rats. The GSH levels in HD treatment seem to be lowered than C+ because the side effect of high intake protein which may disturb GSH synthesis in liver.
Key words : Snakehead fish extract (SHE), diabetic rats, MDA, GSH