HAMEDSOURIAN-REYHANI POUR, RAMIN KHAJAVI*, MOHAMMAD ESMAEIL
YAZDANSHENAS, PAYAM ZAHEDI AND MOHAMMAD MIRJALIL
Textile Chemistry Engineering, Department of Textile Engineering, Yazd Branch, Islamic Azad University,
Yazd, Iran
*(e-mail : rkhajavi@gmail.com; Mobile : 00989312107490)
(Received : June 24, 2019; Accepted : August 20, 2019)
ABSTRACT
In this study, three-layered nanofibrous samples on the basis of cellulose acetate (CA), containing tetracycline hydrochloride (TC) as a middle layer and poly (vinyl alcohol) (PVA) as lateral layer were prepared using electrospinning method. To investigate the morphology, drug release, cytotoxicity and antibacterial properties as well as hydrophilicity of the samples scanning electron microscopy (SEM), ultraviolet visible (UV-Vis) spectrophotometry, thiazolyl blue (MTT), bacterial count and water contact angle were carried out. SEM images showed a uniform morphology with a diameter distribution of 160-190 nm. On the other hand, tetracycline hydrochloride from the three-layered nanofibers was gradually released owing to the poly (vinyl alcohol) gelation, and the release profile followed the Higuchi equation. The MTT test also revealed that the surface of polymer nanofibrous samples, even in the presence of antibiotic, had a good potential for cell viability. Subsequently, the growth inhibitory of E. coli and S. aureus was 89 and 97%, respectively, with the water contact angle approximately 43%, thereby exhibiting the capability of the samples in biomedical applications such as wound dressing.
Key words : Drug release, electrospinning, nanofibers, cellulose acetate, poly (vinyl alcohol)