ZAINAB A. ALI AL-TAHER*
Department of Horticulture and Landscape Engineering, College of Agriculture, University of Basrah, Iraq
*(e-mail: zainab_ali680@yahoo.com; Mobile: 00960 77056 50027)
(Received: March 11, 2023; Accepted: May 2, 2023)
ABSTRACT
This research was carried out in the wooden canopy of the Department of Horticulture and Landscaping at the College of Agriculture on narcissus bulbs of different weights 30, 40, 50 and 60 g. After the seedlings reached the appropriate size, they were sprayed with the prepared concentrations of gibberellins 0, 50 and 100 mg/l. Protein was extracted from dried paper samples using Phenyl methane sulfonyl fluoride (PMSF), and protein movements were performed using the Slab-Electrophoresis technique on a polyacrylamide gel. Using a specialized application called PhotoCapt Mw (version17), the molecular weights of the proteins were calculated. There were variations among all of the study’s circumstances, with the protein bundles’ specifications varying in terms of their size, area and height. All the bulbs shared same molecular weight as the first protein bundle, which amounted to 180.732 Kilo Dalton. All bulbs of different weights also participated in the same molecular weight of the second protein package, which recorded 122.101 Kilo Dalton. The protein bundle counts varied from 4 to 6 depending on the age of the bulb and the gibberellin concentration. Five protein bundles were present in most treatments, and six protein bundles were only in two treatments (bulbs of 50 g + 100 gibberellins and bulbs of 60 g+50 (GA3). Moreover, four protein packages in three treatments: (bulbs 30 g + 50 gibberellins, bulbs 40 g + 50 gibberellins and bulbs 50 g + 50 gibberellins.
Key words : Bulbs, gibberellin, molecular weights, narcissus, protein patterns