REHAB A. MOHAMED*, FATMA F. ABDEL HAMID, DAWOUD F. HABIB, NADIA A. MOHAMED, WAGDY K. B. KHALIL, Medical Biochemistry Department, National Research Centre, Dokki, Giza, Egypt
*(e-mail : roroanas30@yahoo.com; Mobile : +200 12231 19879)
(Received : September 06, 2022; Accepted : September 13, 2022)
ABSTRACT
Drug-induced kidney injury is one of the causatives of acute kidney failure. Amifostine loaded silica
nano emulsion was synthesized using water/oil emulsion with the help of ultra-sonication waves. gp1
normal control (NC) injected with saline daily for one month, gp2 injected with particles of silica nano
emulsion (SiNPs) in a dose of 150 mg/kg/day for one month, gp 3 injected twice with cisplatin in a dose
of 20 mg/kg body weight to induce nephrotoxcity, gp 4 injected with particles of silica nano emulsion of
amifostine (SiNPs@AMF) in a dose of 150 mg/kg/day for one month and gp 5 injected with particles of
silica nanoemulsion of amifostine (SiNPs@AMF) in dose of 150 mg/kg/day for three weeks then injected
with two doses of (cis) (20 mg/kg, day after day) followed by amifostine nanoemulsion injection to
complete one month. Results demonstrated that HPLC analysis of serum homocysteine and tissue
inhibitor metalloproteinase-1 (TIMP-1), osteopontin and RANTS levels showed a significant elevation in
Cis group in comparison with NC group and down regulation of the expression of nephrin mRNA and
podocin mRNA strongly in ne phrotoxic group. Ne phrotoxic animals protected with amifostine
nanoe mulsion re corde d a le ss significant incre ase in se rum homocyste ine , tissue inhibitor
metalloproteinase-1 (TIMP-1), osteopontin and (RANTS) levels with enhancement expression of nephrin
mRNA and podocin mRNA compared to NC.
Key words : Podocyte, nephrotoxicity, silica nanoemulsion, amifostine, cisplatin, nephrin