G. SANDEEP*, T. ARUMUGAM1, G. J. JANAVI, T. ANITHA, K. SENTHIL AND A. LAKSHMANAN
Department of Vegetable Science, Horticultural College and Research Institute, Periyakulam-625 604 (Tamil
Nadu), India
*(e-mail: sandeepguna1995@gmail.com; Mobile: 63800 24258; 87787 41136)
(Received : October 1, 2022; Accepted : November 16, 2022)
ABSTRACT
Moringa (Moringa oleifera Lam.) is an incredible plant with a storehouse of essential nutrients like
protein, vitamins, minerals and phytochemicals such as tannins and flavonoids. From the qualitative
screening, phytochemical was highly present in hydroethanolic extracts. The recovery of these
biomolecules could be achieved by advanced novel extraction methods like Microwave-assisted extraction.
The main aim of this experimental trial was to optimize with a range of three independent factors
including power (500W-700 W), temperature (30-50°C) and extraction time (20-40 min) consisting of two
levels of dependent variables like extraction yield (EY) and total phenol content (TPC) using Response
Surface Methodology (RSM) constructed by Central Composite Design (CCD) with 20 runs. The optimized
extraction method recorded 14.64 to 17.65% of extraction yield and 63.36 to 76.40 mg GA/ gram of total
phenol content. As a result of the optimization of microwave-assisted extraction, recovery of biomolecules
from the dried moringa leaves was achieved by increasing the extraction temperature with the microwave
power of the instrument.
Key words : Moringa, microwave-assisted extraction, biomolecules, response surface methodology, ethanolic extraction