ZAHRAA R. SHAMSEE*, FATIMAH A. SABRY AND AHMED F. ALSHANON
Al-Turath University College, Medical Laboratory Techniques Department, Baghdad, Iraq
*(e-mail: phd.zahraa89@gmail.com; Mobile: +96 91452 56324)
(Received: January 2, 2023; Accepted: February 11, 2023)
ABSTRACT
The present study examined the potential antioxidant activity of purified Lantadene A bind with nano silver extracted from Lantana camara after purification of LA using thin-layer chromatography, silica gel chromatography and high-performance liquid chromatography. UV visible spectra result showed high absorption at 422 wavelengths by lantadene silver nanoparticles. The characterization of nano particles were measured by field scanning electron microscope, UV and atomic forces microscope. Lantadene-Ag increased significantly scavenging activity and it ranged between 70-80% in DPPH and ABTS assay comparing with ascorbic acid. At the same time 200-100 µg/ml of lantadene-Ag showed antioxidant properties and increased significantly reactive oxygen species in hepG2 cell line.
Key words : AgNps, Lantana camara, lantadene A, hepG2 cell line