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Detection and Molecular Characterization of blaVEB and blaCTX-M-1 Genes Producing Imipenem-resistant Klebsiella pneumonia from Birds in Hillah City, Iraq

Biology Department, College of Science for Women, Babylon University, Iraq
*(e-mail:; Mobile: 964 78027 80807)
(Received: December 20, 2022; Accepted: January 30, 2023)


The tool of the current investigation was to assess the frequency of Enterobacteriaceae from fecal samples
of local birds in Hillah city during the period from February, 2022 to the end of April, 2022. Thirty-six per
cent isolates were identified as Enterobacteriaceae when K. pneumonia demonstrated higher frequency 14
(14%) followed by E. coli 10 (10%), Proteus spp. 6 (6%), K. oxytoca 3 (3%) and Enterobacter spp. 3 (3%). Disk
diffusion test was employed to check antimicrobial susceptibility profile of Enterobacteriaceae. All bacterial isolates displayed highest resistant level (100%) for ampicillin, cloxacillin, amoxicillin-clavulanic acid, ceftazidime, ceftriaxone, cefepime and aztreonam. Lower resistance was observed to levofloxacin and imipenem. Genotypic detection of blaVEB and blaCTX-M-1 ESBLs was investigated by PCR technique among imipenem-resistant K. pneumoniae isolates. Six (100%) and two (33.33%) isolates had blaVEB and blaCTX-M-1 gene, respectively.
Key words : Antibiotics resistance, Klebsiella pneumoniae, ESBLs, blaVEB, blaCTX-M-1, local birds, PCR