ASEEL J. MOHAMMAD*, MAY T. FLAYYIH AND INAM B. FALIH
General Directorate of Education in Baghdad, Al-karkh Firsest, Iraq
*(e-mail: la_aaaa@yahoo.com; Mobile: 00964 79016 52623)
(Received: May 15, 2022; Accepted: June 17, 2022)
ABSTRACT
A phenolic compound in coffee has antimicrobial activities and anti-inflammatory effects. This study
investigated firstly the antibacterial effect of coffee phenolic extract through the agar-well diffusion
method, and secondly, the role of coffee in the mice hepatic and small bowel (ileum section) post
Escherichia coli infection with apoptotic cell detection. The agar well diffusion method was used for the
concentrations of 50, 100 and 150 mg/ml, respectively. Results showed that the minimum inhibitory
concentration of coffee alcohol extract was 150 mg/ml with an inhibition zone of 19 mm. The results of
histopathology showed fewer pathological changes in the hepatic and small bowel (ileum section) tissues
in a treated grouping. In contrast, the results of apoptosis showed lower apoptotic activity in treated
groups in the hepatic and small bowel tissues.
Key words : Arabic coffee, Escherichia coli, histopathology, apoptosis, mice