RAND MUHAMMED ABDUL-HUSSEIN AL-HUSSEINI*, RAED ALI HUSSAIN AND ALI MOHAMMED ABED
Department of Biology, Faculty of Science, University of Kufa, Al-Najaf Governorate, Iraq
*(e-mail : rand.alhusseini@uokufa.edu.iq; Mobile : 009647725728195)
(Received : August 17, 2019; Accepted : October 18, 2019)
ABSTRACT
Breast cancer is the most common type of cancer among women. Increasing the expression of certain genes due to polymorphism increases the risk of breast cancer. Genomic DNA was isolated from 100 Iraqi women, 75 with confirmed breast cancer and 25 with normal breast tissues were considered as control group. These paraffin block samples were collected during the period August 2018 until April 2019 from the laboratories of histopathology in An Najaf city. ARMS-PCR (tetra primer technique) was used for genotyping Rrp1B gene 1307T>C (rs9306160) polymorphism in breast cancer patients and control samples. The genotype distribution of the 1307T>C (rs9306160) single-bp polymorphisms of Rrp1B gene showed significant difference (P<0.05) between controls (TT : n=5, 20%; TC : n=6, 24%; CC : n=14, 56%) and breast cancer patients (CC : n=52, 69.33%; CT : n=15, 20% and TT : n=8, 10.66%). The association of Rrp1B 1307T>C polymorphism and pathological features of the breast cancer cases was analyzed. There were significant associations between Rrp1B 1307T>C polymorphism and histological grade, TNM staging and lymph node metastases of breast cancer cases (P<0.05).
Key words : Rrp1B gene, breast cancer, Iraq, rs9306160 polymorphism