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Biochemical and Molecular Characterization of Endophytic Bacteria from Root Nodules of Mungbean (Vigna radiata)

REKHA SANSANWAL*, UMANG AHLAWAT, PRIYANKA BATRA, LEELA WATI AND PREETI KAUSHIK
Department of Microbiology, Chaudhary Bansi Lal University, Bhiwani-127 021 (Haryana), India
*(e-mail : rsansanwal90@gmail.com)
(Received: May 28, 2022; Accepted: June 16, 2022)

ABSTRACT

Bacterial endophytes are endosymbiotic in nature and employ advantageous effects on host plant as
stimulation of plant growth, nitrogen fixation and induction of resistance to plant pathogen. In the
present study, mungbean nodule samples were collected from farm area during summer and kharif
season to isolate the endophytes. In total, 41 endophytes were isolated using Tryptone soya agar (TSA)
and the majority of endophytes were found to be IAA producers, phosphorus solubilizer, potassium
solubilizer and ACC utilizers and trove of many other plant growth traits. On the basis of morphological,
biochemical and molecular characterization, four plant growth promoting endophytes found were Bacillus
flexus strain PM1217, Bacillus amyloliquefaciens strain PM1218, Beijerinckia fluminensis strain PM1219
and Pseudomonas sp. strain PM1220. These non-rhizobial endophytes can significantly influence the
mungbean growth and provide a strong basis for further development of these strains as efficient
biofertilizers to attain the desired yield in fields.
Key words : Mungbean, non-rhizobial, endophytes, TSA, plant growth promotion (PGP)