SHALINI VERMA, MONIKA KUMARI, VIKAS YADAV1 AND PUJA YADAV*
Department of Microbiology, Central University of Haryana, Mahendergarh-123 031 (Haryana), India
*(e-mail: pujayadav@cuh.ac.in; Mobile:86859 95379)
(Received: March 1, 2023; Accepted: April 2, 2023)
ABSTRACT
Several studies have been carried out in bacteria for the identification of gene(s) or protein(s) involved
in biofilm formation for the development of anti-biofilm targets but with limited success. However, information about the involvement of proteins during biofilm formation in GBS is poorly understood. Therefore, in the present study, by using mass spectrometry, a comparative proteomics study was performed for the GBS cultured during planktonic and biofilm conditions to identify proteins expressed exclusively during the biofilm development. Two hundred and four proteins were found commonly present in both the planktonic and the biofilm stages. However, 192 and 98 proteins were exclusively detected in planktonic and biofilm stages, respectively. Out of these, 98 proteins present in the biofilm stage were associated with cellular metabolism, translations, cell wall and cell membrane synthesis machinery. Moreover, only 20 proteins (out of 98) have been characterized so far in GBS for their role in pathogenesis and therefore, further characterization of the remaining 78 proteins in biofilm formation and pathogenesis may open avenues for designing new-age drugs to combat GBS burden world-wide.
Key words : Streptococcus agalactiae, biofilm, antibiotic resistance, ESI-LC-MS/MS, proteomics