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Studies on Phytochemical Properties and Antibacterial Activity of Moringa oleifera Flower Extract

Department of Food Technology and Nutrition, Lovely Professional University, Phagwara-144 411 (Punjab),
*(e-mail:;; Mobile: 89882 27729)
(Received: March 22, 2023; Accepted: April 26, 2023)


The Moringa oleifera plant is a well-known and commonly utilized herbal remedy for a variety of contagious
and non-contagious medical disorders. In this study, results revealed the presence of various phytochemicals: alkaloids, phenolic and flavonoid compounds, tannins, saponins and terpenoids. HPLC analysis revealed the presence of various phytochemicals such as gallic acid, quercetin and rutin. The goal of this study was to analyze the phytochemicals and to assess the antibacterial effects of M. oleifera flower extract (MOFE) against two common microorganisms: E. coli ATCC25922 and S. aureus ATCC33591. M. oleifera flower extract exhibited antibacterial effects against the two tested bacteria E. coli and S. aureus with the diameter of the zone of inhibition 11 and 9 mm, respectively. Five different concentrations (1000, 500, 250, 125, 62.5 mg/ml) of M. oleifera flower extract were prepared in 5% DMSO (Dimethyl sulfoxide) and tested for the antibacterial effect by using the agar disc diffusion method. Minimum inhibitory concentrations were found to be 250 mg/ml. M. oleifera had high antibacterial action against bacterial pathogens that were pathogenic and virulent, and it may be a source of yet another efficient and practical antibacterial antibiotic.
Key words : Contagious, phytochemicals, dimethyl sulfoxide, minimum inhibitory concentration, zone of inhibition