INTISAR GHANIM ABDULWAHHAB*
Biology Department, College of Education for Women, Tikrit University, Tikrit, Iraq
*(e-mail : dr.en79@tu.edu.iq; Mobile : 00964 77066 30310)
(Received : February 16, 2022; Accepted : March 18, 2022)
ABSTRACT
Toxoplasma gondii is considered an obligatory intracellular parasite that causes toxoplasmosis. This
study included 100 infertile women. Serum and whole blood were taken from the included subjects. The
DNA was extracted from the whole blood by using a commercial kit. Real-time PCR was done for all the
samples to detect the presence of T. gondii. PCR was done for the positive samples to amplify a target
sequence within the rop-17 gene. The PCR product was sequenced to reveal the variations within the
rop-17 gene. The ELISA test was done to measure the serum level of SOD and GPX. The real time-PCR
showed 43% frequency of the samples. The conventional PCR was used to amplify the rop-17 gene in the
positive samples but showed a successful amplification in 92% of the real-time PCR positive samples.
The results of the sequences showed different variations within the rop-17 gene. GPX and SOD were
both higher in patients (93.76±9.6 and 89.6±9.4, respectively) than in control (76.5±31.4 and 85.6±7.43,
respectively).
Key words : GPX, SOD, rop-17 gene, variations, sequence, real time, PCR