SHAIMAA N. MIZIL*, EKHLAS A. J. ELKAABY AND MAHER Z. F. AL-SHAMMARY
Department of Biology, College of Education for pure science Ibn AL-Haitham, University of Baghdad, Baghdad, Iraq
*(e-mail: Shaymaa.m1979@gmail.com; Mobile: +96 77261 22081)
(Received: February 06, 2023; Accepted: April 10, 2023)
ABSTRACT
The study was performed at the laboratory of tissue culture to evaluate the effect of gamma doses, namely, 0, 20 or 40 Gy and mannitol stress at 0, 40 and 60 g/l on growth parameters on callus produced from Golden Sunrise cherry tomato in vitro. The results indicated significant increase in callus fresh and dry weight (232.9 and 33.6 mg) at 20 Gy and 40 g/l mannitol. Although there was a significant increase in the accumulation of CHO at a rate of 5.203 mg/g in the presence of 60/g mannitol, on the contrary, the results displayed a great reduction (1.089 mg/g dry weight) of potassium accumulation in callus exposed to 60 g/l mannitol. Furthermore, 100% of plant regeneration from stressed callus in most of the hormonal combinations in the presence of (0, 40 g/l) mannitol and (0, 20 and 40 Gy) gamma doses. In conclusion, the results of this study recommended low doses of gamma radiation to improve plant regeneration from stressed callus of Golden Sunrise cherry tomato. It was suggested that these dosages could be used as anti-stress factors in tomatoes.
Key words : Cherry tomato, CHO, K+, mannitol, plant regeneration, potassium