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Effect of the Glycoside Extract of Nerium oleander L. Flowers on the Arabidopsis thaliana Colombia-0

INAS SABAH MOHAMMED SHAREIF, SAHAR A. A. MALIK AL-SAADI* AND HUSSEIN K. AL-KAABY
Department of Biology, College of Science, University of Basrah, Basrah, Iraq
*(e-mail : saharmalik2010@gmail.com; Mobile : +964 77121 66577)
(Received : April 23, 2022; Accepted : May 25, 2022)

ABSTRACT

The effect of Nerium oleander flower glycoside extract on Arabidopsis thaliana (Colombia-0) was investigated
at different concentrations viz., 0, 2, 4, 6 and 10 g/ml. According to biochemical and morphological
features, glycoside compounds reduced leaf area, highest value, leaf number and plant height. Chlorophyll
pigments had maximum value in the control (4.2 mg/g) and then decreased in the treatments. The effect
of glycoside chemical compounds varied between the control and treatment groups. The number of
glycoside compounds of A. thaliana revealed 34 chemical compounds, whereas the treatment recorded 20,
12, 9 and 5 chemical compounds in 2, 4, 6 and 10%, respectively. The main compounds in control
treatment were 8-Nonene-1-nitrile (25.46%), 2.alpha., 4a.beta., 8a.beta. Decahydro-2-naphthalenol was
13.38%. As well as in 2% was 2.alpha., 4a.beta., 8a.beta. Decahydro-2-naphthalenol (28.78%), 8-Nonene1-nitrile was 14.59%. Similarly, in 4% concentration treatment main compound was 8-Nonene-1-nitrile
32.56%, pyrazine, tetramethyl 26.62%. Main compound in 6% concentration 8-Nonene-1-nitrile was
36.85%. The analysis of the glycoside extract of the 10% treatment showed the highest value ceten
27.41%, and 8-Nonene-1-nitil by 20.07%.
Key words : Biochemical, chlorophyll, Nerium oleander, Arabidopsis thaliana, GC-MS analysis