YAZI ABDULLAH JASSIM*, MARWA HUSAIN ABDULLA MOHI AL-KHAFAJI AND SURA I. A. JABUK
Department of Biology, College of Science, Babylon University, Babylon, Iraq
*(e-mail : yaziabdalla2014@gmail.com; Contact : +964 78097 42785)
(Received : March 15, 2022; Accepted : April 21, 2022)
ABSTRACT
Microbial enzymes in natural environments have a role in biochemical activities and the biotechnological
potential of microbes. During this study, 150 isolates from different environments, soil, water and
plants were studied, and their ability to produce different enzymes (caseinase, gelatinase, amylase,
carboxymethyl cellulase, and esterase) was examined. Ninety per cent of the isolates produced at least
one enzyme. The isolated microbes from the three environments produced the highest percentage of
caseinase enzyme. The soil microbes were also characterized by high production of cellulase and amylase
enzyme. The microbes of the aquatic environment were characterized by high production of the enzyme
esterase compared with the microbes of the plants that had a high efficiency in the production of the
cellulase enzyme. The study showed that optimum incubation time for enzymes production was 72 h
and 7.5 pH. The ability of most microbes to produce enzymes used in biotechnology exploration produced
was affected by the type of microbial environment of the microorganism.
Key words : Caseinase, gelatinase, amylase, carboxymethyl cellulase, esterase, optimum conditions