NUR HIDAYAT CHE MUSA, HANIZA HANIM MOHD ZAIN*, HUSNI IBRAHIM, NOR NASIBAH MOHD JAMIL AND M. T. A. MOHAMMED
Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong
Malim, Perak, Malaysia
*(e-mail : haniza@fsmt.upsi.edu.my; Mobile : 0060 19512 7712)
(Received : February 15, 2022; Accepted : March 3, 2022)
ABSTRACT
The purpose of this study was to examine the toxic impact of aqueous extract of Aquilaria subintegra leaf
(AEAS) on body weight (b. w.), relative organ weight (ROW), fatality rate, and sperm variables in male
ICR mice. In acute toxicity study, the treated animals received a single dose of AEAS at 2000 mg/kg
b. w., while in sub-acute toxicity study; the treated groups received 50, 100, 150, 200, 500 and 1000 mg/
kg b.w. of AEAS orally for 21 days consecutively. The control group received normal saline only. All
experimental animals were survived until the end of the experiment except in the treated group that
received AEAS 1000 (20%). Mild sedative effects manifested as sleepiness in treated groups receiving
higher doses of AEAS during the first two to three hours after administration. The number of abnormal
sperm significantly decreased in AEAS 2000 (acute) and AEAS 100, 150 and 1000 (sub-acute) compared
to control. However, sperm motility significantly increased in almost all AEAS treated groups. Thus,
AEAS was non-toxic to the treated mice and had therapeutic potential to improve sperm quality and
enhanced breeding rate, specifically in mice.
Key words : Aquilaria subintegra leaf, aqueous extract, toxicity studies