MOH. AWALUDIN ADAM*, RAMLI, ACH KHUMAIDI AND AGOES SOEGIANTO **
Faculty of Science and Technology, University of Ibrahimy, Situbondo, Indonesia
*(e-mail : firstname.lastname@example.org; Mobile : +62 87865867060; **email@example.com; Mobile : +62
The objective of this study was to analyze the histopathological picture of the gills of gambusia fish exposed to cadmium. Fish were exposed to different levels of cadmium, namely, treatment A : 1 ppm; treatment B : 0.1 ppm; treatment C : 0.01 ppm; treatment D : 0.001 ppm; treatment E : 0.0001 ppm and control treatment. After 96 h of treatment, clinical symptoms were observed in the gills. The gills of cadmium exposed fish were then fixed using 10% phosphate-buffered formalin (NBF) for 24-48 h, cut and stained with hematoxylin-eosin (HE). The tissue analysis was done descriptively by looking at the tissue changes caused by cadmium exposure. Consistent histopathological changes were found in gills
exposed to cadmium.
Key words : Gambusia, histopathology, cadmium, acute toxicity