RAMAN BALA*, JITENDER SINGH LAURA AND VIJAY SINGH BENIWAL
Department of Environmental Science, Maharshi Dayanand University, Rohtak-124 001 (Haryana), India
*(e-mail : nandwalraman72@gmail.com)
(Received : October 2, 2019; Accepted : December 9, 2019)
ABSTRACT
Simmondsia chinensis (Link) Schneider (family – Simmondsiaceae) is a valuable seed oil plant of the Indian Thar Desert. The present investigation was mainly focused to develop an efficient method using shoot tip explants of mature female Simmondsia chinensis. Among the different concentrations (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) of BAP and KIN tested, highest per cent (95.8%) of shoot bud induction with maximum
number of shoot buds (4.12 shoots/explant) was observed on MS medium supplemented with 1.5 mg/l of BAP with 20 mg/l adenine sulphate. For multiplication and elongation of shoots, the regenerated shoot buds were sub-cultured on the medium containing 1.5 mg/l BAP (optimum concentration) with combination of IBA, NAA and IAA (0.1, 0.5, 1.0 and 1.5 mg/l). The highest number of shoots (8.64 shoots/explant) on
MS medium containing 1.5 mg/l BAP+0.5 mg/l NAA with a proliferation rate of 98.5% were obtained with average shoot length of 5.10 cm. Among the various concentrations used, the highest frequency (95.3%) of root induction was noticed on 3.0 mg/l IBA with 200 mg/l activated charcoal. Well rooted plantlets were successfully hardened and acclimatized with 80% survival rate. The acclimatized plants were found similar as the mother plants. The present in vitro propagation method for female S. chinensis could be useful for large scale production of female plants for successful establishment and commercial cultivation.
Key words : Adenine sulphate, female, shoot tip explants, Simmondsia chinensis