NENCY KATRODIYA, NIKITA PATEL, SWETAL PATEL, DAVID ADEDAYO ANIMASAUN, STEPHEN OYEDEJI, DHANJI, P. RAJANI, SHIVA SHANKARAN CHETTIAR AND R. KRISHNAMURTHY*
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Mahuva Road, Bardoli, Surat-394 350 (Gujarat),
India
*(e-mail : krishnashanti@gmail.com, krishnamurthy@utu.ac.in; Mobile : 98253 49279)
(Received : November 15, 2021; Accepted : January 11, 2022)
ABSTRACT
Urinary tract infection (UTI) is a major public health concern, especially in women, across the globe.
Among the uropathogens, E. coli is most implicated in UTI infected patients. Urine samples were collected,
cultured and subcultured using different media to determine the morphology and biochemical properties
of the bacteria in the samples. DNA samples from the pure cultures were amplified, sequenced and
subjected to BLAST analysis. Isolates identified in the urine samples belonged to the genus Escherichia,
Klebsiella, Proteus, Enterobacter, Staphylococci and Brevibacilli. The presence of Escherichia marmotae and
Brevibacillus panacihumi was confirmed from the result of the BLAST. The study implicated E. marmotae
and B. panacihumi in UTI infection in the studied population. Further study is encouraged to analyze the
virulence genes responsible for their pathogenicity.
Key words : : Urinary tract infection, uropathogens, molecular identification, brevibacillus