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Optimization of Brassica oleracea var. Pusa Snowball Using Seedling Explants for the in vitro Regeneration Protocol

Amity Institute of Biotechnology, Amity University, Sector 125, Noida-201 313 (Uttar Pradesh), India
*(e-mail :; Mobile : 99108 34670)
(Received : October 20, 2022; Accepted : January 04, 2023)


Cauliflower is an important Cole crop grown in India but the attack of insects-pests hampers the production
of the crop. Generation of the transgenic crop resistant to insects can serve to be a solution for the problem. Therefore, establishment of an optimized in vitro regeneration protocol of the Indian cauliflower is a pre-requisite, hence, two explants, namely, cotyledon and hypocotyls were obtained from the seedlings of the Indian cauliflower variety Pusa Snowball K-1. Results showed that the shoot regeneration from hypocotyls
explants on media containing 1 mg/l BAP along with the combination 0.1 mg/l IAA was best suited and for root regeneration 1.5 mg/l IBA was found to be most effective. Further, standardized protocol complemented for Agrobacterium-mediated transformation against the insect Diamondback moth i.e. the DBM.
Key words : In vitro regeneration, Indian cauliflower, hypocotyl, cotyledon, diamond back moth