Search for content, post, videos

Sugarcane Bagasse Pretreatment with Deep Eutactic Solvent and Saccharification by Crude Cellulase Enzyme

Department of Agricultural Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110 (U. P.), India
*(e-mail :; Mobile : 8171382761)
(Received : November 6, 2019; Accepted : January 10, 2020)


The pretreatment of sugarcane bagasse using deep eutectic solvent (DES), choline chloride (ChCl) : urea for effective delignification and its comparison with acid and alkaline pretreatment was demonstrated. ChCl : urea (1 : 2) worked as a very effective extraction media for lignin extraction from sugarcane bagasse at 45°C temperature and 10% solid loading. It was shown that nearly 44.5% of lignin was separated in a
single step. The DES pretreated sugarcane bagasse showed the maximum reducing sugar yield of 322±11mg/g and saccharification efficiency of 24.5±1.1% after enzymatic saccharification with crude cellulase enzyme
in 48 h at 10% solids loading.
Key words : Deep eutectic solvents, pretreatment, saccharification, sugarcane bagasse