VITA TJANDRA, YULI WIDIYASTUTI, AMALIA TETRANI SAKYA AND AHMAD YUNUS*
Department of Agronomy, Graduate School, Universitas Sebelas Maret, Surakarta, Indonesia
*(e-mail : yunus.uns7@yahoo.com; Mobile : +6281548673060)
(Received : July 5, 2019; Accepted : August 10, 2019)
ABSTRACT
Coleus atropurpureus (L.) Benth. is known as one of the types of medicinal plants. However, there has been no standardization of techniques for increasing flavonoid production levels in coleus plants until today. The aim of this study was to determine the effect of cattle manure dosage (nutrient stress) and various accessions of coleus plant [Coleus atropurpureus (L.) Benth.] on the flavonoid content and also to discover the accession of coleus plant and cattle manure dosage (nutrient stress) that improve the flavonoid content. This research was conducted from July 2018 to September 2018 at Screen House, Jumantono, Sebelas
Maret University Surakarta. The research method used a complete randomized design with four accessions of coleus plants (coarse purple, fine purple, red and green leaves). The various dosages of manure (nutrient stress) were 0, 1, 2.5 and 5 t/ha. The data obtained were analyzed by the analysis of variance (ANOVA) and followed by Duncan’s multiple range test at 5% level. Analysis of total flavonoid content was done by the AlCl3 method using a UV-Vis spectrophotometer, analysis of N levels on coleus leaf tissue was done using the Kjeldahl method and analysis of TLC was done by using TLC densito metric method with nhexane : ethyl acetate (6 : 4) as mobile phase. The result showed that each accession had different response on application of cattle manure and the highest flavonoid contents were accessions 2 (6.32%) and cattle
manure dosage 2.5 t/ha (5.38%).
Key words : Coleus atropurpureus, accession, nutrient stress, flavonoid content, TLC