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The Effects of Phytohormones and Yeast Extract on Callus Development of Medicinally Important Endangered Plant, Rauwolfia serpentina (L.)

Sachin Kumar Verma, Pratima Bhagat, Smriti Yadav, Danswrang Goyary, Gajendra Kumar Aseri and Neeraj Khare
Amity Institute of Microbial Technology, Amity University Rajasthan, NH-11C, Jaipur-303 002 (Rajasthan), India
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(Received : November 26, 2020; Accepted : December 29, 2020)


Rauwolfia serpentina (L.) is a hub of more than 120 medicinally important terpenoid indole alkaloid mainly reserpine, ajmalicine, ajmaline, yohimbine, etc. Plant tissue culture is a potential technique for the large-scale production of these pharmaceutically important alkaloids. In the present study, significantly exorbitant callus induction efficiency was recorded in MS media fortified with 2 mg/l 2,4-D and 1 mg/l BAP as compared to other tested combinations within three weeks of culture with 100% efficiency. Further, different concentrations of yeast extract were evaluated for the efficient development of callus. Significantly higher biomass was observed on 0.75 g/l yeast extract supplemented culture medium. The present study will be useful in efficient callus development of R. serpentina.
Key words : Rauwolfia serpentina, yeast extract, growth additive