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Whole Cell Protein Profiling of Salmonella Typhimurium Isolates from Poultry

Vipin Khasa, Suman Chaudhary, Vipul Chaudhary, Pamela Singh, Shashi Chawla and Aman Kumar
Department of Biotechnology, Deenbandhu Chhotu Ram University, Murthal, Sonipat-131 006 (Haryana), India
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(Received : November 12, 2020; Accepted : January 15, 2021)


Salmonellosis is one of the major bacterial diseases affecting poultry world-wide and has public health significance. This study was carried out on nineteen S. Typhimurium isolates from broiler population of Haryana to determine the whole cell protein profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). All isolates (n=9) were found to carry more than 30 KDa protein bands, where maximum number of isolates shared major bands of 37.3, 46, 51.2, 78.1 and 150 KDa. Isolates studied were found closely related with slight variation in same serovar. SBT-4, 5, 6, 7 and 16 were found to carry different protein profile, which suggested that this technique can be used for epidemiological studies
Key words : Salmonella, whole cell protein, poultry, protein profiling