MAMTA SHARMA*, DUSHYANT KUMAR, PANKAJ THAKUR AND ENZA TORINO
School of Biological and Environmental Sciences, Shoolini University, Solan-173 229 (H. P.), India
*(e-mail : mamta131526@gmail.com; Mobile : 70185 78162)
(Received : July 13, 2021; Accepted : September 05, 2021)
ABSTRACT
Current focus of nanotechnology is to develop environmentally safe methodologies for formulation of
nanoparticles. The presented study reports a rapid, simplistic green synthesis of environmentally safe
AgNPs capped and stabilized with leaf extracts of Mentha piperita and Berberis lysium. The formation and
stability of reduced AgNPs were monitored by TEM and UV Spectroscopy. The stability was evaluated at
varying pH and different concentration of NaCl at temperature range of 20-80°C. Antimicrobial activity of
the AgNPs was accomplished by disc diffusion method against both S. typhi and E. coli. The highest
antimicrobial activity of AgNPs synthesized by M. piperita and B. lyceum was found against S. typhi (700
µg) and E. coli (700 µg), respectively, which could be due to high content of berberin and menthol in
extract. It was inferencing that leaf extract of M. piperita and B. lycium proved good bio-reductant for biosynthesis of AgNPs which may prove versatile for assorted biomedical and pharmaceutical applications.
Novelty of this present study was that plant extracts were very cost-effective, eco-friendly, and effective
substitute for bio-synthesis of AgNPs for antibacterial activity and cytotoxic activity.
Key words : : AgNPs, UV-visible spectroscopy, FT-IR, TEM, antimicrobial, Escherichia coli, Salmonella typhi