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Enhanced Callus Growth of Medicinally Important Rauwolfia serpentina (L.) through Supplementation of Phytohormones and L-glutamine

Sachin Kumar Verma, Pratima Bhagat, Abhishek Bhargava, Anushka Chatta, Danswrang Goyary, Gajendra Kumar Aseri and Neeraj Khare
Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur-303 002 (Rajasthan), India
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(Received : November 27, 2020; Accepted : January 18, 2021)


Rauwolfia serpentina (L.) is a woody perennial important medicinal shrub belonging to Apocynaceae family. In the present study, an efficient reproducible protocol for callus development from leaf explants was standardized using L-glutamine. L-glutamine enhanced callus biomass by 1.56 times at a concentration of 150 mg/l as compared to control treatment. The present study incorporated a step in the improved protocol for the callus development of R. serpentina by adding L-glutamine.
Key words : : R. serpentina, phytohormones, L-glutamine