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Induction of Kidney Lesions in Rats by Streptococcus pyogenes and Evaluate the Herbal Component Activity of Symphytum officinale Extract

Department of Biology, College of Education for Pure Science, Kirkuk University, Kirkuk, Iraq
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(Received: January 2, 2023; Accepted: February 11, 2023)


The current work was aimed at investigating the activity of Symphytum officinale extract to treat the kidney lesions induced by Streptococcus pyogenes. The throat swabs were collected from children patients in Azadi teaching hospital during March to July 2022. Twenty-four male albino rats were placed into four groups each of six rats; negative group which received orally normal saline (0.5 ml/kg) BW; positive group in which injected (intraperitoneal) 105 CFU of S. pyogenes; extract group which orally received S. officinale 150 mg/kg BW and treated group in which injected (intraperitoneal) by 105 CFU of S. pyogenes and treated by S. officinale 150 mg/kg. There were significant variations between groups in oxidative stress and antioxidant enzymes then the control group. The MDA levels in bacterial infected group were significantly higher, while GSH and catalase levels in bacterial infected group were significantly lower. The treated group’s levels of MDA, GSH and catalase revealed non-significant changes after utilizing S. officinale extract. In the histological study, the microscopic examination of the kidney’s cross section revealed various lesions following infection, including damage to the glomerulus, tubule cell deterioration, lymphocyte infiltration and thickening of blood vessel walls. After treatment with extract, cross sections showed semi-normal structure of glomerulus and semi-normal form and diameter of convoluted urinary tubules. In conclusion, S. officinale extract can be efficient for decreasing population of S. pyogenes and treating infections for severe anti-oxidative effects.
Key words : Streptococcus pyogenes, Symphytum officinale, kidney, oxidative stress