AMANKELDI KURBANOVICH SADANOV*, ALMAGUL KHAMITOVNA KHASSENOVA,
SAULE TLEKOVNA DAUGALIYEVA, ZERE ZHAIKOVNA TURLYBAYEVA AND AISHA ZHENISBAEVNA SULTANOVA
LLC “Research and Production Center for Microbiology and Virology” Bogenbai Batyr Street 105, Almaty, 050010, Kazakhstan
*(e-mail : k.anara@mail.ru; Contact : 7+7272+918497)
(Received : March 25, 2020; Accepted : May 21, 2020)
ABSTRACT
Colonies of actinomycetes were taken from the samples of the rhizosphere of the eryngo plants (Eryngium planum) and isolated into pure cultures. Their antagonistic properties were studied using laboratory collection
strains of gram-positive (Bacillus subtilis ATCC 6633) and gram-negative (Comamonas terrigena ATCC 8461) test microorganisms using the agar block method. Actinomycetes with high antagonistic activity were chosen and the strains were selected using chemical mutagenesis. As a result of chemical mutagenesis, highly active mutant strains were obtained and their genetic stability was studied using successive subcultures on an agarized growth medium with activity control for each sub-culture. A mutant strain of a
natural broad-spectrum antibiotic C5 2XM2 was obtained, which was promising for further studies. The species affiliation of the actinomycetes culture was established using molecular genetic analysis based on the study of the 16S rRNA gene sequence.
Key words : Actinomycetes, antibiotics, chemical mutagenesis, mutagen, molecular genetic analysis