RENU JAGDISH AND KIRAN NEHRA*
Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal (Sonipat)-
131 039 (Haryana), India
*(e-mail: kirannehra.bt@dcrustm.org; Mobile : 92552 66011)
(Received: November 17, 2022; Accepted: December 21, 2022)
ABSTRACT
The phyto-mediated green methodology using different parts of a plant has been observed to be a simple
and novel method, having an edge over the other methods to synthesize nanoparticles. The plant biomolecules act as capping and stabilizing agents for synthesizing nanoparticles with the release of toxic-free by-products and also enhance their antimicrobial ability against a wide range of pathogens. In this direction, the present study involved the green synthesis of zinc oxide nanoparticles by employing Elaeocarpus ganitrus plant leaf extract. The various abiotic parameters (concentration of the metal salt, plant leaf extract volume, temperature and pH) were standardized and optimized to obtain the most effective nanoparticles. The synthesis of standardized ZnO nanoparticles was affirmed by UV-Vis spectroscopy, which depicted a sharp absorption peak at 361 nm. The XRD analysis revealed the crystalline nature and hexagonal wurtzite phase of the synthesized ZnO nanoparticles. Upon evaluation of the antibacterial response of these nanoparticles against gram-positive pathogenic bacteria, they were observed to exhibit zone of inhibitions as 27.9±0.63 and 27±0.47 mm against the tested pathogens, thus exhibiting their strong antibacterial ability. Hence, from the present study, it could be concluded that the green synthesis methodology can be used effectively for synthesizing nanoparticles possessing potent antibacterial responses against pathogenic bacterial isolates.
Key words : Antibacterial ability, Elaeocarpus ganitrus, nanoparticles, phytoconstituents, XRD