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Physico-chemical Parameters Optimization for Phycobiliprotein Production from Cyanobacteria

Manju Bala Bishnoi, Minakshi Lalit, Meenakshi, Sandeep Kumar and Namita Singh
Microbial Biotechnology Laboratory, Department of Bio and Nano Technology, Guru Jambheshwar University of
Science and Technology, Hisar-125 001 (Haryana), India
*(e-mail :; Mobile : 9416928883)
(Received : January 11, 2021; Accepted : March 14, 2021)


Cyanobacteria are good source of a natural blue coloured pigment called phycobiliprotein (PBP). A statistical method called response surface methodology (RSM) was used for optimizing different physicochemical parameters for the increased production of phycobiliproteins by cyanobacterial isolate 13MNS2014; RSM used Box-Behnken design for optimizing three parameters, named, temperature, light intensity and pH, each with three levels. Biomass production (in respect of ratio of protein/chl. a) and total phycobiliprotein content were used to analyze the optimum physicochemical parameter conditions. The optimized conditions observed for cyanobacterial isolate 13MNS2014 were 30°C temperature, 7.5pH and 2000 lux light intensity at which the highest value of pigments and biomass production was obtained. At optimized conditions, PBP production increased upto 40%. This study signified the cyanobacterial isolate 13MNS2014 potential of enhanced PBP production under optimized conditions which may have possible industrial importance as PBP can be a good candidate to replace the chemically synthesized colorants or dyes.
Key words : : Cyanobacteria, phycobiliprotein, response surface methodology, colorants, physicochemical parameters