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Total Flavonoid Content of Three Echinacea purpurea Accessions with Watering Interval

Rina Puji Lestari, Yuli Widiyastuti, Samanhudi and Ahmad Yunus
Department of Agronomy, Graduate School, Sebelas Maret University (UNS), Jl. Ir. Sutami 36A, Kentingan,
Surakarta, 57126, Central Java, Indonesia
*(e-mail : yunus.uns7@yahoo.com; Mobile : +6281548673060)
(Received : January 22, 2021; Accepted : March 10, 2021)

ABSTRACT

The provision of different watering intervals is an approach in optimizing the production of specific total
Echinacea purpurea flavonoids. The purpose of this study was to determine the total flavonoid content on three accessions of E. purpurea and the effect of various watering intervals on the total flavonoid content on three accessions of E. purpurea. Planting was carried out in Jumantono experimental field, Faculty of Agriculture, Sebelas Maret University in March-August 2020. The planting of E. purpurea was done in a complete randomized factorial design (CRD) with the first factor as the accession (A) with three levels (A1, A2and A3). The second factor was watering interval with four levels : once a day (C1), once every three days (C2), once every five days (C3) and once every seven days (C4). Flavonoid analysis was conducted at the Laboratorium Research and Development Center for Medicinal Plants and Traditional Medicines (B2P2TOOT), Tawangmangu. The accession 2 showed the highest total flavonoid content of 0.300% and extract yield 0.107%. Watering interval affected all three accessions.
Key words : : Accsession, Echinacea purpurea, flavonoid total, secondary metabolites, watering interval