Renu Yadav, Anita Rani Santal and Nater Pal Singh
Centre for Biotechnology, Maharshi Dayanand University, Rohtak-124 001 (Haryana), India
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(Received : April 21, 2021; Accepted : June 13, 2021)
In the advancing horizons, proteomic analysis has become a vital tool for sketching the biological architecture of wheat. The experiment was conducted at the three-leaf stage. The root proteins were focused; the seedlings were subjected to 0 and 300 mM of NaCl for 48 h. Liquid chromatography coupled with mass spectrometry (LC-MS/MS) technique was applied to the isolated proteins. An LC-MS/MS proteomic analysis of salinity responsive biological processes viz., intracellular protein transport, biosynthetic process, lipid transport and glycolysis of KH-65 and PBW-373 was carried out. The genotypic response towards salinity was found to be a significant function of change in protein expressions. The label-free quantitative method brought significantly valid results. The data showed genotype-specific variations in protein expression patterns that contributed to different levels of salinity tolerance. Proteins, namely, VHS domain-containing protein, Clathrin heavy chain, vacuolar protein sorting-associated protein 41 homologs and non-specific lipid-transfer protein, expressed in the intracellular transport process and lipid transport process, respectively, were the reason for salt tolerance in KH-65.
Key words : : Triticum aestivum L., salt stress, root proteomics, LC-MS/MS