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Proteomic Analysis of Some Salt Responsive Biological Processes in Wheat (Triticum aestivum L.) under Salt Stress

Renu Yadav, Anita Rani Santal and Nater Pal Singh
Centre for Biotechnology, Maharshi Dayanand University, Rohtak-124 001 (Haryana), India
*(e-mail :; Mobile : 8950309162)
(Received : April 21, 2021; Accepted : June 13, 2021)


In the advancing horizons, proteomic analysis has become a vital tool for sketching the biological architecture of wheat. The experiment was conducted at the three-leaf stage. The root proteins were focused; the seedlings were subjected to 0 and 300 mM of NaCl for 48 h. Liquid chromatography coupled with mass spectrometry (LC-MS/MS) technique was applied to the isolated proteins. An LC-MS/MS proteomic analysis of salinity responsive biological processes viz., intracellular protein transport, biosynthetic process, lipid transport and glycolysis of KH-65 and PBW-373 was carried out. The genotypic response towards salinity was found to be a significant function of change in protein expressions. The label-free quantitative method brought significantly valid results. The data showed genotype-specific variations in protein expression patterns that contributed to different levels of salinity tolerance. Proteins, namely, VHS domain-containing protein, Clathrin heavy chain, vacuolar protein sorting-associated protein 41 homologs and non-specific lipid-transfer protein, expressed in the intracellular transport process and lipid transport process, respectively, were the reason for salt tolerance in KH-65.
Key words : : Triticum aestivum L., salt stress, root proteomics, LC-MS/MS