LEANDER CORRIE, SACHIN KUMAR SINGH*, MONICA GULATI, NARENDRA KUMAR PANDEY, RUBIYA KHURSHEED, ANKIT AWASTHI, JASKIRAN KAUR AND SUKRITI VISHWAS
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara-144 411 (Punjab), India
*(e-mail: singhsachin23@gmail.com, sachin.16030@lpu.co.in; Mobile: 88978 24213)
(Received: June 20, 2022; Accepted: August 12, 2022)
ABSTRACT
An analytical method was developed by employing ultrafast liquid chromatography and a C-18 reverse
phase column for estimating and validation of curcumin (CMN). The mobile phase was made up of
acetonitrile and water in a 90 : 10 v/v ratio. The flow rate was set at 1 ml/min. A chromatogram of CMN
was detected at a wavelength of 420 nm. The retention time for CMN was found to be 3.14 min. The
developed method was found to be linear in the range of 2-10 µg/ml with a regression coefficient of 1.
The method developed was validated according to the Q2 (R1) requirements of the International Conference
on Harmonization (ICH). The percentage recovery was in the range of 95 to 105%, indicating that the
procedure was accurate. The relative standard deviation (RSD) as a percentage was found to be less
than 2%, indicating the methods precision. The limit of detection was found to be 77.7 ng/ml and the
limit of quantification at 235.6 ng/ml. The method developed was proven to be robust because the
response did not change significantly with changes in flow rate or wavelength. The method was used to
estimate drug loading and release from a self-nano emulsifying drug delivery system. The percent drug
loading of CMN in the produced liquid self nano emulsifying drug delivery system (SNEDDS) formulation
was 99.95%. At the end of 60 min, dissolution studies revealed a 78.78% CMN release in water.
Key words : Curcumin, SNEDDS, RP-HPLC, validation, analysis