Reema Ningthoujam and Harish Kumar Dhingra
Department of Biosciences, School of Liberal Arts and Sciences (SLAS), Mody University of Science and Technology,
Lakshmangarh-332 311, Sikar (Rajasthan), India
*(e-mail : harishdhingra2000@gmail.com; Mobile : 9461536233)
(Received : December 8, 2020; Accepted : February 10, 2021)
ABSTRACT
A variety of waste materials can act as a substrate for the production of bioethanol by Saccharomyces cerevisiae. Currently, bioethanol is produced from sugar or starch-based raw materials that are scarce. The ethanol produced from the cellulosic wastes at the cheaper cost will be a great advantage to the oil companies in producing blended petrol at a cheaper price. The cellulosic wastes like industrial paper wastes, which otherwise create lots of environmental pollution on burning, have been successfully used as a substrate for bioethanol production in this experimental study. This work utilized industrial paper waste as a substrate for ethanol production. It was found that bioethanol production was greatly influenced by the culture conditions and media components. The potential strains were isolated from over-ripened fruits such as apples, plums, grapes, etc. The incubation period of 32 h and maintaining the temperature and pH at 27°C and 4, respectively, was found optimum for maximum bioethanol production. Ethanol production was measured after a week of fermentation with S. cerevisiae; and the highest yield of ethanol was found to be 15.6%.
Key words : Yeasts, ethanol, blending, substrate, fermentation